Human LAIR-1 Protein, Mouse IgG2a Fc Tag

Leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1) is constitutively expressed on the majority of human peripheral blood mononuclear leukocytes. LAIR-1 or CD305 is a transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs. LAIR-1 recruits SHP-1 and SHP-2 phosphatases upon activation, and cross-linking of the LAIR-1 antigen on natural killer (NK) cells results in strong inhibition of NK cell–mediated cytotoxicity. Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation.Diseases associated with LAIR1 include Chronic Active Epstein-Barr Virus Infection and Palindromic Rheumatism.

Product Specifications

Background

Specifications

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 42.3 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q6GTX8-1

Expression Region

Gln 22 - His 163

Host

HEK293

Target

LAIR-1

Conjugation

Unconjugated

Tag

C-mFc (mIgG2a)

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Human LAIR-1, Mouse IgG2a Fc Tag (Cat. No. LA1-H5253) with a linear range of 10-78 ng/mL (QC tested).