Necrocide 1

Necrocide 1, a necrosis by sodium overload (NECSO) inducer, is a selective transient receptor potential melastatin 4 (TRPM4) agonist with an EC50 of 306.3 nM for human TRPM4. Necrocide 1 triggers TRPM4-dependent necrotic cell death through the induction of sodium influx. Necrocide 1 induces hallmarks of immunogenic cell death incurring calreticulin (CALR) exposure, ATP secretion and high mobility group box 1 (HMGB1) release. Necrocide 1 can be used for the study of breast and prostate cancer[1][2][3].

Product Specifications

CAS Number

[1247028-61-0]

UNSPSC

12352005

Target

Eukaryotic Initiation Factor (eIF) ; Reactive Oxygen Species (ROS) ; TRP Channel

Type

Reference compound

Related Pathways

Cell Cycle/DNA Damage; Immunology/Inflammation; Membrane Transporter/Ion Channel; Metabolic Enzyme/Protease; Neuronal Signaling; NF-κB

Applications

Cancer-programmed cell death

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/necrocide-1.html

Concentration

10mM

Purity

98.84

Solubility

DMSO : 100 mg/mL (ultrasonic)

Smiles

O=C1NC2=C(C=CC(OC)=C2C)[C@]1(C3CCCCCC3)C4=CC=C(O)C=C4

Molecular Formula

C23H27NO3

Molecular Weight

365.47

References & Citations

[1]Christensen MK, et, al. Synthesis and antitumor effect in vitro and in vivo of substituted 1,3-dihydroindole-2-ones. J Med Chem. 2010 Oct 14;53 (19) :7140-5.|[2]Christensen MK, et al. Synthesis and antitumor effect in vitro and in vivo of substituted 1,3-dihydroindole-2-ones. J Med Chem. 2010 Oct 14;53 (19) :7140-5. |[3]Fu W, et al. Persistent activation of TRPM4 triggers necrotic cell death characterized by sodium overload. Nat Chem Biol. 2025 Aug;21 (8) :1238-1249. |[4]Zhang J, et al. Necrocide 1 mediates necrotic cell death and immunogenic response in human cancer cells. Cell Death Dis. 2023 Apr 5;14 (4) :238.