CheKine™ Micro Hepatic Lipase (HL) Activity Assay Kit

Hepatolipase (HL) is a type of lipolytic enzyme synthesized in liver parenchymal cells. It exists on the surface of endothelial cells in the perisinusoidal space of the liver and on the microvilli surface of liver cells in the perisinusoidal space. It can hydrolyze triglycerides (TG) and phospholipids (PL) in various lipoproteins, causing changes in the size and density of various lipoprotein particles. When the HL and its activity in the plasma increase, it can lead to low-density lipoprotein (LDL) in the plasma The increase of the level accelerates the occurrence and development of atherosclerosis. HL hydrolysis α-Naphthyl acetate to produce α-Naphthol, which can form a purple red azo compound with fixed blue B salt, has a characteristic absorption peak at 595 nm, and its color depth is positively correlated with HL activity within a certain range.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Storage at ４°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

• If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples.