Fluorescamine
Fluorescamine is a spirocyclic compound that is non-fluorescent. Fluorescamine reacts rapidly with primary amine groups in proteins under alkaline conditions to generate products with strong fluorescence (Ex/Em: 390/475 nm) . Fluorescamine can be used to detect amine-containing compounds, including amino acids, peptides, and proteins[1][2].
Product Specifications
CAS Number
38183-12-9
Product Name Alternative
Ro 20-7234
UNSPSC
12171500
Hazard Statement
H315, H319, H335
Target
Fluorescent Dye
Type
Dye Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/Fluorescamine.html
Purity
98.0
Solubility
DMSO : 100 mg/mL (ultrasonic)
Smiles
O=C1C2(OC(C3=C2C=CC=C3)=O)OC=C1C4=CC=CC=C4
Molecular Formula
C17H10O4
Molecular Weight
278.26
Precautions
H315, H319, H335
References & Citations
[1]Udenfriend S, et al. Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range. Science. 1972 Nov 24;178 (4063) :871-2. |[2]Lorenzen A, et al. A fluorescence-based protein assay for use with a microplate reader. Anal Biochem. 1993 Oct;214 (1) :346-8.
Shipping Conditions
Room Temperature
Storage Conditions
4°C (Powder, protect from light)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported
Available Sizes
Frequently Asked Questions
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