Fluorescamine

Fluorescamine is a spirocyclic compound that is non-fluorescent. Fluorescamine reacts rapidly with primary amine groups in proteins under alkaline conditions to generate products with strong fluorescence (Ex/Em: 390/475 nm) . Fluorescamine can be used to detect amine-containing compounds, including amino acids, peptides, and proteins[1][2].

Product Specifications

CAS Number

[38183-12-9]

Product Name Alternative

Ro 20-7234

UNSPSC

12171500

Hazard Statement

H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/Fluorescamine.html

Purity

98.0

Solubility

DMSO : 100 mg/mL (ultrasonic)

Smiles

O=C1C2(OC(C3=C2C=CC=C3)=O)OC=C1C4=CC=CC=C4

Molecular Formula

C17H10O4

Molecular Weight

278.26

Precautions

H315, H319, H335

References & Citations

[1]Udenfriend S, et al. Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range. Science. 1972 Nov 24;178 (4063) :871-2. |[2]Lorenzen A, et al. A fluorescence-based protein assay for use with a microplate reader. Anal Biochem. 1993 Oct;214 (1) :346-8.