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ChIP Lysis Buffer (Biotechnology Grade)

<strong>ChIP Lysis Buffer (Biotechnology Grade)</strong>_x000D_ <table width="1028">_x000D_ <tbody>_x000D_ <tr>_x000D_ <td width="285">Catalog number:</td>_x000D_ <td width="743">B2010092</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Lot number:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Expiration date:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Volume/Weight</td>_x000D_ <td>50 mL</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>pH</td>_x000D_ <td>N/A</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Supplied as</td>_x000D_ <td>1X</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Appearance</td>_x000D_ <td>Clear solution</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Applications</td>_x000D_ <td>ChIP Lysis Buffer is a useful for chromatin Immunoprecipitation. Highly stable and highly reproducible.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Shelf-Life</td>_x000D_ <td>1 year from date of manufacture</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Storage:</td>_x000D_ <td>Keep at 2-8°C</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Keywords:</td>_x000D_ <td>ChIP buffer, chip buffer composition, Chromatin Immunoprecipitation buffer, lysis chip, chip cell lysis buffer</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Grade</td>_x000D_ <td>Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered on a 0.22 um.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>References</td>_x000D_ <td width="743">1: Mohammed H, Taylor C, Brown GD, Papachristou EK, Carroll JS, D'Santos CS._x000D_ Rapid immunoprecipitation mass spectrometry of endogenous proteins (RIME) for_x000D_ analysis of chromatin complexes. Nat Protoc. 2016 Feb;11(2):316-26.2: Mundade R, Ozer HG, Wei H, Prabhu L, Lu T. Role of ChIP-seq in the discovery_x000D_ of transcription factor binding sites, differential gene regulation mechanism,_x000D_ epigenetic marks and beyond. Cell Cycle. 2014;13(18):2847-52._x000D_ _x000D_ 3: Cam HP, Whitehall S. Chromatin Immunoprecipitation (ChIP) in_x000D_ Schizosaccharomyces pombe. Cold Spring Harb Protoc. 2016 Nov 1;2016(11)._x000D_ _x000D_ 4: Rehimi R, Bartusel M, Solinas F, Altmüller J, Rada-Iglesias A. Chromatin_x000D_ Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples. J Vis_x000D_ Exp. 2017 Aug 29;(126):56186._x000D_ _x000D_ 5: Wade JT. Mapping Transcription Regulatory Networks with ChIP-seq and RNA-seq._x000D_ Adv Exp Med Biol. 2015;883:119-34._x000D_ _x000D_ 6: Giaimo BD, Ferrante F, Borggrefe T. Chromatin Immunoprecipitation (ChIP) in_x000D_ Mouse T-cell Lines. J Vis Exp. 2017 Jun 17;(124):55907._x000D_ _x000D_ 7: Nakato R, Shirahige K. Recent advances in ChIP-seq analysis: from quality_x000D_ management to whole-genome annotation. Brief Bioinform. 2017 Mar_x000D_ 1;18(2):279-290._x000D_ _x000D_ 8: Mitra S, Rai LS, Chatterjee G, Sanyal K. Chromatin Immunoprecipitation (ChIP)_x000D_ Assay in Candida albicans. Methods Mol Biol. 2016;1356:43-57._x000D_ _x000D_ 9: Schmidl C, Rendeiro AF, Sheffield NC, Bock C. ChIPmentation: fast, robust,_x000D_ low-input ChIP-seq for histones and transcription factors. Nat Methods. 2015_x000D_ Oct;12(10):963-965._x000D_ _x000D_ 10: Orlando DA, Chen MW, Brown VE, Solanki S, Choi YJ, Olson ER, Fritz CC,_x000D_ Bradner JE, Guenther MG. Quantitative ChIP-Seq normalization reveals global_x000D_ modulation of the epigenome. Cell Rep. 2014 Nov 6;9(3):1163-70._x000D_ _x000D_ 11: Liu F, Feng X, Ma W. Analysis of Telomere Proteins by Chromatin_x000D_ Immunoprecipitation (ChIP). Methods Mol Biol. 2017;1587:205-214._x000D_ _x000D_ 12: Mbogning J, Tanny JC. Chromatin Immunoprecipitation of Histone Modifications_x000D_ in Fission Yeast. <a href="https://pubmed.ncbi.nlm.nih.gov/27854023/">Methods Mol Biol.</a> 2017;1528:199-210.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Related Products</td>_x000D_ <td><a href="https://moleculardepot.com/product-category/buffers/">Biochemical Buffers and Solutions</a></td>_x000D_ </tr>_x000D_ </tbody>_x000D_ </table>

Product Specifications

Short Description

Catalog Number: B2010092 (50 mL) ChIP Lysis Buffer is a useful for chromatin Immuno-precipitation. Filter-sterilized, this buffer is highly stable and highly reproducible. Custom bulk orders are available upon request.

Weight

2.6

Length

3.3

Width

1.2

Height

1.2

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