Anti-Ubiquitin Antibody

Rabbit Polyclonal Antibody specific to Ubiquitin

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Ubiquitin Rabbit Polyclonal Antibody

Gene Name

UBC

Gene ID

7316

NCBI Gene ID

Accession Number

NP_066289.3

Host

Rabbit

Reactivity

Human, Mouse, Rat, Rabbit, Guinea Pig, Hamster, Sheep, Chicken, Monkey, Bovine, Canine, Porcine, Fish, Yeast, E. Coli, Drosophila, Xenopus

Immunogen

Native bovine ubiquitin conjugated to KLH.

Target Antigen

Polyubiquitin-C [Cleaved into: Ubiquitin]

Target

Ubiquitin

Clonality

Polyclonal

Type

Antibody

Applications

WB, IP, IHC

Field of Research

Apoptosis

Purification Method

Purified by protein A affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: Use at 1-5ug/mL. A band of ~10kDa is detected. Immunohistochemistry: use at 1-10ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

Ubiquitin

Formulation

PBS, pH 7.2, 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.2

Target Background

Ubiquitin is a highly-conserved, 76-amino acid polypeptide expressed in all eukaryotic cells. Ubiquitination of proteins facilitates targeting of cytosolic and nuclear proteins for degradation by proteasomes. This essential cellular process involves a multi-enzyme cascade of ubiquitin- activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs), and ubiquitin-protein ligases (E3s). Ubiquitination is also involved in internalization and degradation of plasma membrane proteins such as T cell receptor subunits while still associated with membranes of the endoplasmic reticulum.