Beta actin Monoclonal Antibody
Product Specifications
Background
Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells, and commonly used as a loading control for western blot and as a control for qPCR.
Abbreviation
Beta actin
UniProt
P60709
Host
Mouse
Reactivity
Human; Mouse; Rat
Immunogen
Recombinant human Beta actin protein by E.coli
Target
ADLD; lamin B1; Lamin-B1; LMN; LMN2; LMNB; Lmnb1; LMNB1; MGC111419; OTTHUMP00000159218
Clonality
Monoclonal
Clone
3A9
Conjugation
Unconjugated
Applications
WB; IHC; IF
Field of Research
Signal Transduction; Tags and Cell Markers; Isotype/Loading; Controls; Neuroscience; Biochemicals; Cancer; Cardiovascular; Cell Biology; Immunology; Metabolism; Epigenetics and Nuclear Signaling; Stem Cells
Purification
Protein A/G Purification
Concentration
1 mg/mL
Dilution
WB 12000-1:4000; IHC 1:200-1:400; IF 1:200-1:400
Buffer
PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH 7.4
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Calculated Molecular Weight
43 kDa
Observed Molecular Weight
43 kDa
Isotype
IgG1
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