Anti-Smac (CT) Antibody

Rabbit Polyclonal Antibody specific to Smac (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase, Smac

Gene Name

DIABLO

Gene ID

56616

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=DIABLO">DIABLO</a>

UniProt

Q9NR28

Accession Number

NP_063940

Cellular Locus

Mitochondrion

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to aa 225-239 of human Smac (accession no. AAF87716).

Target Antigen

Diablo IAP-binding mitochondrial protein

Target

Smac (CT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IHC-P, IP, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. {PubMed:10929711, PubMed:14523016, PubMed:15200957}.

Additionnal Information

Immunoblotting: use at 1ug/mL. Positive control: Human heart tissue lysate. Immunohistochemistry: use at 5ug/mL These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat Smac (25kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Inhibitors of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases has been identified and designated Smac/DIABLO. Smac is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates the cytochrome c/Apaf- 1/caspase-9 pathway. The N-terminal amino acids of Smac are required for binding to IAPs and for activation of caspases. Smac is expressed in a variety of human and mouse tissues.