Hifair™ Precision sgRNA Synthesis Kit

The Hifair™ Precision sgRNA Synthesis Kit uses a T7 RNA polymerase to efficiently transcribe spCas9 sgRNA. This kit contains a specific sequence that is recognized by the Cas9 protein and acts as a scaffold, and part of this sequence can partially overlap with the user-designed specific sequence of interest. After annealing, complementary strands are formed, which are filled by DNA polymerase. The resulting dsDNA template for transcription is generated. Using the reagents provided and user-designed specific DNA sequences, this kit can obtain 20-100 μg of functional sgRNA in 4 hours in a single tube reaction.