Anti-KLK11/Hippostatin Antibody

Rabbit Polyclonal Antibody specific to Hippostatin/KLK11

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

hK11, EC 3.4.21.-, Hippostasin, Serine protease 20, Trypsin-like protease [Cleaved into: Kallikrein-11 inactive chain 1; Kallikrein-11 inactive chain 2]

Gene Name

KLK11

NCBI Gene ID

UniProt

Q9UBX7

Cellular Locus

[Isoform 1]: Secreted.; [Isoform 2]: Golgi apparatus.

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to aa residues 68-82 (YIVHLGQHNLQKEEG) of human

Target Antigen

Kallikrein-11

Target

KLK11/Hippostatin

Clonality

Polyclonal

Type

Antibody

Applications

WB, ELISA

Field of Research

Cancer research

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. {PubMed:10872828, PubMed:16467084}.

Additionnal Information

Immunoblotting : use at 1:500 dilution. <br><br>Positive control: LNCaP cell lysate

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human hippostatin/KLK11 (35 kDa) which has 86% sequence homology with mouse and rat.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Hippostatin, also known as KLK11, is a kallikrein-like serine protease, which has two alternatively spliced isoforms, brain- type and prostate-type. Hippostatin plays a role in the prostate, including reproductive and/or tumorigenic functions. Elevated serum levels of KLK11 were found in 70% of women with ovarian cancer and in 60% of men with prostate cancer. Analysis of the KLK11 biomarker in serum may aid in the diagnosis and monitoring of ovarian and prostatic carcinoma.