Human Beta-NGF Protein, premium grade
Human Beta-NGF Protein, premium grade (BEF-H5214) is expressed from human 293 cells (HEK293) . It contains AA Ser 122 - Ala 241 (Accession # P01138) .
Product Specifications
Background
Nerve growth factor beta (beta-NGF) is a neurotrophic factor that plays critical role in the development and maintenance of sensory and sympathetic neurons. The active form of human beta-NGF is is a noncovalently disulfide-linked homodimer, which is secreted and signals through the low affinity nerve growth factor receptor (LNGFR) and the tropomyosin receptor kinase A (TrkA) to activate PI3K, Ras, and PLC signaling pathways.
Specifications
This protein carries no "tag". The protein has a calculated MW of 13.5 kDa. The protein migrates as 14-15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
P01138
Expression Region
Ser 122 - Ala 241
Host
HEK293
Target
Beta-NGF
Conjugation
Unconjugated
Tag
Native
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
0.01 EU per μg
Purity
90%
Bioactivity
Immobilized Human Beta-NGF Protein, premium grade (Cat. No. BEF-H5214) at 1 μg/mL (100 μL/well) can bind Human TrkA (33-417), Mouse IgG2a Fc Tag (Cat. No. TRA-H5259) with a linear range of 1-16 ng/mL (QC tested).
Format
Powder
Buffer
20 mM NaAC, 150 mM NaCl, pH5.5
Additives
Trehalose
Molecular Weight
13.5 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
20ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/BEF-H5214.pdf
Species
Human
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
