HCMV (strain AD169) Glycoprotein B (gB), His Tag (MALS verified)

HCMV Glycoprotein B (gB) Protein (Strain AD169), His Tag is the ectodomain of HCMV (strain AD169) glycoprotein B (gB) which contains AA Ser 25 - Phe 706 (Accession # P06473 (Y155G, I156H, Y157R, Y206H, W240A, L241T, Y242H, R456S, R459S) ) . The recombinant protein is expressed from human 293 cells (HEK293) with a polyhistidine tag at the C-terminus. Mutations Y155G, I156H, Y157R, Y206H, W240A, L241T, Y242H and R456S, R459S are introduced to stabilize the trimeric postfusion state of HCMV gB protein and abolish the furin cleavage site, respectively.

Product Specifications

Background

Human cytomegalovirus is a species of the Cytomegalovirus genus of viruses, which in turn is a member of the viral family known as Herpesviridae or herpesviruses. It is typically abbreviated as HCMV or, commonly but more ambiguously, as CMV. CMV Virus Envelope Glycoportein B (CMV-GB) can be cleaved into glycoprotein GP55. Envelope glycoprotein that plays a role in host cell entry, cell to-cell virus transmission, and fusion of infected cells. CMV-GB may be involved in the initial attachment via binding to heparan sulfate together with the gM/gN complex that binds heparin with higher affinity. Furthermore, CMV-GB can interact with host integrin ITGB1, PDGFRA and EGFR that likely serve as postattachment entry receptors. Also, CMV-GB participates in the fusion of viral and cellular membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 79.6 kDa. The protein migrates as 90-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P06473 (Y155G, I156H, Y157R, Y206H, W240A, L241T, Y242H, R456S, R459S)

Expression Region

Ser 25 - Pro 706

Host

HEK293

Target

Glycoprotein B/gB (HCMV)

Conjugation

Unconjugated

Tag

C-10xHis

Source

HCMV

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized HCMV (strain AD169) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H4) at 1 μg/mL (100 μL/well) can bind Anti-gB(HCMV)-antibody-SM5-1,Human IgG1(AM330) with a linear range of 0.1-4 ng/mL (QC tested).