Collagen Type II, Monoclonal Antibody (Clone M2139)

This antibody (clone 2139) recognizes the conformational J1 epitope on the triple-helical structure of the native Collagen Type II molecule. The J1 epitope on mouse collagen consists of residues 551–564. It cross-reacts with mouse, rat, human, bovine, and chicken. Collagen type II, encoded by the COL2A1 gene, is a crucial component of cartilage and plays a vital role in maintaining joint integrity. In mouse models of arthritis, such as collagen-induced arthritis, COL2A1 is studied for its role in cartilage degradation, a key feature of conditions like rheumatoid arthritis and osteoarthritis. Research on COL2A1 helps in understanding arthritis mechanisms and de veloping new treatments. M2139 binds to the triple helical J1 epitope on collagen II. Binding to collagen II epitopes was determined in bead-based flow immunoassay as median fluorescence intensity (MFI) . Triple helical (Gly-Pro- Hyp) x11 is a synthetic control peptide with 11 repeats of the Gly-Pro-Hyp sequence and mimics the collagen II backbone structure.

It is recommended that the user determine the optimal dilution for their application. The typical starting working dilutions are for functional assays (cell culture) 1:20-1:50 and IHC 1:100.

Collagen Type XI, Monoclonal Antibody, 100ug; ACC1, Monoclonal Antibody, 100ug; ACC4 (IgG1), Monoclonal Antibody, 100ug; ACC4 (IgG2b), Monoclonal Antibody, 100ug; ACPA, Monoclonal Antibody (Clone E4NG), 100ug