KRISHGEN CAR/TCR Transgene Copy Number Quantitation RT-PCR Kit
The KRISHGEN CAR/TCR Transgene Copy Number Quantitation Kit is a qPCR based assay to quantify the copy number of target genes in cell therapy products. It utilizes multiplex qPCR technique to detect DNA sequences related to the integration or expression function on the transfer plasmid and Single Copy Gene (SCG) in human cells. The average copies of target gene per cell can be calculated in the samples, such as CAR or TCR gene copy numbers. This kit is specific, accurate and universal for lentiviral vector applications.
Product Specifications
Short Description
Assay Type
Real Time PCR assay. Instrument compatability to Thermo, Biorad, Roche, Qiagen.
Assay Principle
Sample Type
Cell Culture Supernatant and other biological samples
Detection Range
CAR/TCR: 23.3 - 23.3 million copies / ul SCG: 233 - 23.3 million copies / ul
Sensitivity
LOQ = CAR/TCR: 23.3 copies / ul | SCG: 52.85 copies / ul LOD = 2.33 copies /ul
Shipping Conditions
-20 Degrees Celsius, Dry Ice
Storage Temperature
Specificity
The primers in the kit are specific for the analyte to be measured using the standards available in the kit for quantification of the results.
Frequently Asked Questions
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