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KRISHGEN rcAAV-5/N Quantitation RT-PCR Kit

The KRIBIOLISA Bioprocess Assay Kits available enable researchers to estimate and measure the amount of process analyte left during the production process. These assays can help identify or characterize the residual analyte which is important in early process, downstream purification and final product release. This Assay kit is designed to facilitate the identification and characterization of analytes used in production and purification. Assay Features include: - Ready to use protocol with ease of use components - Standardisation and High Reproducibility - Lot to Lot Consistency - Accuracy and Precision Validated against seven points for a Gold Ring Standard Quality - the benchmark sign for Krishgen quality. The kits are used for assessing the specific biomarker in samples analyte which may be cell culture supernatant and other biological preparations. Unlike competitor assays available, we offer pre-coated ready-to-use assays to ensure high level of reproducibility and accuracy and also offer custom assay development which is process specific to comply with FDA needs from our ISO13485 certified factory and labs.

Product Specifications

Short Description

Assay Range: 2 million copies / ul | Sensitivity: 0.4 copy / ul Assay Range: | Sensitivity: Availability: 3 - 4 Weeks | Pack Size: 200 reactions Availability: | Pack Size:

Assay Type

Real Time PCR assay. Instrument compatability to Thermo, Biorad, Roche, Qiagen.

Assay Principle

Real-time PCR monitors the accumulation of PCR product (amplicon) cycle-by-cycle using a fluorescent signal that increases proportionally with the amount of amplified DNA.

Sample Type

Cell Culture Supernatant and other biological samples

Detection Range

2 million copies / ul

Sensitivity

0.4 copy / ul

Shipping Conditions

-20 Degrees Celsius, Dry Ice

Storage Temperature

The kit and its components are to be stored as indicated in the IFU (instructions for use) . To ensure quality of your results, if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, well sealed and store at the indicated temperature.

Specificity

The primers in the kit are specific for the analyte to be measured using the standards available in the kit for quantification of the results.

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