Anti-N-WASP (Tyr-256), Phosphospecific Antibody

Our N-WASP (Tyr-256) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat N-WASP (Tyr-256) and is antigen affinity purified. It is great for use in ELISA, WB, ICC.

Product Specifications

Background

Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with higher homology in the functional regions of these proteins. Phosphorylation at serine and tyrosine residues regulates the activity of both proteins. WASP is tyrosine phosphorylated at tyrosine 291 after antigen receptor activation in B-cells and collagen stimulation of platelets. Phosphorylation of the analogous site in N-WASP (Tyr-256) stimulates its activity, reduces nuclear N-WASP, and is required for neurite extension.

Short Description

Synonyms

Neural Wiskott-Aldrich syndrome protein, WASL, WASP

Gene Name

WASL

UniProt

O00401

Modification Site

Tyr-256

Reactivity

Human, Mouse, and Rat

Immunogen

Phospho-N-WASP (Tyr-256) synthetic peptide (coupled to BSA) corresponding to amino acid residues around tyrosine 256 of human N-WASP. The human WASP sequence has a two amino acid difference in the same region surrounding tyrosine 291.

Target

N-WASP (Tyr-256)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, ICC

Field of Research

Cell Signaling, Cytoskeletal Regulation

Format

Antigen Affinity Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylated

Molecular Weight

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Affinity purified rabbit serum

WB Dilution

1:1000

Specificity

This antibody was cross-adsorbed to phosphotyrosine then affinity purified using phospho-N-WASP (Tyr-256) peptide (without carrier) . The antibody detects a 65 kDa* protein corresponding to the molecular mass of phosphorylated N-WASP on SDS-PAGE immunoblots of A431 cells treated with pervanadate. A similar band is observed in pervanadate treated HeLa and endothelial cells. Weak bands are also observed at higher molecular weights after pervanadate treatment. These bands may be due to low cross-reactivity with phosphotyrosine.