Anti-VASP (Thr-278), Phosphospecific Antibody

Our VASP (Thr-278) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat VASP (Thr-278) and is antigen affinity purified. It is great for use in ELISA, WB, ICC.

Product Specifications

Background

Actin filament tethering and bundling are important mechanisms involved in actin superstructure assembly. The ENA/VASP family includes VASP, mena, and Ena-Vasp-like (EVL) . These multidomain proteins localize to the leading edge of filopodia where they associate with AFs, interact with profilin, and compete with capping proteins at the barbed end of AFs. Artificial relocalization of VASP from the plasma membrane to mitochondrial membranes inhibits filopodial formation and axon branching, while deletion of all three ENA/VASP proteins produces defects in cortical axon-tract formation. Regulation of VASP protein activity occurs through phosphorylation at Ser-157, Ser-239, and Thr-278. AMPK phosphorylates Thr-278, leading to impaired actin stress fiber assembly and changes in cell morphology.

Short Description

Synonyms

Vasodilator-stimulated phosphoprotein

Gene Name

VASP

UniProt

P50552

Modification Site

Thr-278

Reactivity

Human, Mouse, and Rat

Immunogen

Phospho-VASP (Thr-278) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding threonine 278 in human VASP. This sequence has significant homology to the conserved site in rat and mouse VASP, but is not conserved in mena or EVL proteins.

Target

VASP (Thr-278)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, ICC

Field of Research

Cell Adhesion Molecules, Cell Signaling, Cytoskeletal Regulation, Extracellular Matrix Proteins

Format

Antigen Affinity Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylated

Molecular Weight

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Affinity purified rabbit serum

WB Dilution

1:1000

Specificity

The antibody detects a 50 kDa* band corresponding to VASP in Western blots of human A431 and HeLa cells, as well as mouse C2C12 cells that have been treated with calyculin A.