Anti-VASP (C-terminal region) Antibody

Our VASP (C-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human VASP (C-terminal region) and is protein a purified. It is great for use in ELISA, WB, ICC.

Product Specifications

Background

Actin filament tethering and bundling are important mechanisms involved in actin superstructure assembly. The ENA/VASP family includes VASP, mena, and Ena-Vasp-like (EVL) . These multidomain proteins localize to the leading edge of filopodia where they associate with AFs, interact with profilin, and compete with capping proteins at the barbed end of AFs. Artificial relocalization of VASP from the plasma membrane to mitochondrial membranes inhibits filopodial formation and axon branching, while deletion of all three ENA/VASP proteins produces defects in cortical axon-tract formation. Regulation of VASP protein activity occurs through phosphorylation at Ser-157, Ser-239, and Thr-278. AMPK phosphorylates Thr-278, leading to impaired actin stress fiber assembly and changes in cell morphology.

Short Description

Our VASP (C-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human VASP (C-terminal region) and is Protein A purified. It is great for use in WB, ICC.

Synonyms

Vasodilator-stimulated phosphoprotein

Gene Name

VASP

UniProt

P50552

Reactivity

Human

Immunogen

Clone (M277) was generated from a recombinant protein that includes amino acids from the C-terminal region of human VASP.

Target

VASP (C-terminal region)

Clonality

Monoclonal

Clone

M277

Conjugation

Unconjugated

Applications

ELISA, WB, ICC

Field of Research

Cell Adhesion Molecules, Cell Signaling, Cytoskeletal Regulation, Extracellular Matrix Proteins

Format

Protein A Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

GOmAb

Molecular Weight

46/50

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein A purified culture supernatant.

WB Dilution

1:1000

Specificity

This antibody detects 46 and 50 kDa* proteins corresponding to the apparent molecular mass of VASP on SDS-PAGE immunoblots of human A431, HeLa, and HUVEC.