Anti-α6-Tubulin (Ser-165), Phosphospecific Antibody

Our α6-Tubulin (Ser-165) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and xenopus α6-Tubulin (Ser-165) and is antigen affinity purified. It is great for use in ELISA, WB.

Product Specifications

Background

Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of a/β-Tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in α- and β-Tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of βIII-Tubulin in vitro, and unphosphorylated Ser-444 may be an early marker for cells of neuronal lineage. Cdk1 can phosphorylate Ser-172 in β-Tubulin during mitosis and this may impair tubulin incorporation into microtubules. In α-tubulin, PKC can phosphorylate Ser-165 leading to increased cell motility in human breast cells.

Short Description

Our α6-Tubulin (Ser-165) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus α6-Tubulin (Ser-165) and is antigen affinity purified. It is great for use in WB.

Synonyms

Alpha 6 Tubulin, Tubulin A6

Gene Name

TUBA1C

UniProt

Q9BQE3

Modification Site

Ser-165

Reactivity

Chicken, Human, Mouse, Rat, and Xenopus

Immunogen

Phospho-α6-Tubulin (Ser-165) synthetic peptide (coupled to KLH) corresponds to amino acid residues around serine 165 of human α6-Tubulin. This sequence is well conserved in most α-tubulin isoforms from most eukaryotic species, but is not conserved in β-Tubulin.

Target

α6-Tubulin (Ser-165)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Field of Research

Cell Signaling, Cytoskeletal Regulation

Format

Antigen Affinity Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylated

Molecular Weight

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Affinity purified rabbit serum

WB Dilution

1:1000

Specificity

This antibody was cross-adsorbed to unphosphorylated α6-Tubulin (a.a. 160-169) peptide before affinity purification using α6-Tubulin (Ser-165) peptide (without carrier) . The antibody detects a 50 kDa* protein corresponding to the molecular mass of α-Tubulin on SDS-PAGE immunoblots of recombinant a1-tubulin phosphorylated with PKCα. This reactivity is not observed after lambda phosphatase treatment.