Anti-Trop-2/TACSTD2 (Extracellular region) Antibody

Our Trop-2/TACSTD2 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Trop-2/TACSTD2 (Extracellular region) and is protein g purified. It is great for use in ELISA, WB, ICC, IP.

Product Specifications

Background

Trop-2 (TACSTD2) is a transmembrane glycoprotein found on invasive trophoblast cells and in several epithelial type cancer cells. Trop-2 has an extracellular domain with EGF thyroglobulin type-1 repeats, a transmembrane domain and a short cytoplasmic tail with a HIKE domain containing a PIP2 binding site. This glycoprotein functions in many signaling pathways including interaction of its extracellular domain with integrin β1 to regulate FAK signaling, interaction of its transmembrane domain with claudin 1 and claudin 7 during tight junction formation, and regulation of intracellular calcium release by its PIP2 binding and activation of the ERK/MAPK pathway. These Trop-2 functions may be important during tumor proliferation, metastasis and invasion.

Short Description

Our Trop-2/TACSTD2 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Trop-2/TACSTD2 (Extracellular region) and is Protein G purified. It is great for use in WB, ICC, IP.

Synonyms

Tumor-associated calcium signal transducer 2; Trop-2; Membrane component chromosome 1 surface marker 1; GA733-1; TACSTD2; M1S1; TROP2

Gene Name

TACSTD2

UniProt

P09758

Reactivity

Human

Immunogen

Clone M005 was generated from a proprietary antigen related to the extracellular region of human Trop-2 from the A431 epidermoid carcinoma cell line.

Target

Trop-2/TACSTD2 (Extracellular region)

Clonality

Monoclonal

Clone

M005

Conjugation

Unconjugated

Applications

ELISA, WB, ICC, IP

Field of Research

Cell Adhesion Molecules, Cell Signaling, Growth Factor Signaling, Kinase Signaling, Oncoproteins

Format

Protein G Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

GOmAb

Molecular Weight

50-65

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein G purified culture supernatant.

WB Dilution

1:1000

IP Dilution

1:100

Specificity

Clone M005 detects a 50-65 kDa* band corresponding to the molecular mass of Trop-2 on SDS-PAGE immunoblots of native human A431, MDA-MB-231, and MCF-7 cell lysates. The antibody also detects denatured Trop-2, but with less affinity. Lower dilutions of the antibody should be used for denatured western blot. Clone M005 can be used for western blot, immunocytochemistry, ELISA, and immunoprecipitation, as well as for detecting live unfixed cells.