Anti-Stat3 (N-terminal region) Antibody

Our Stat3 (N-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Stat3 (N-terminal region) and is protein a purified. It is great for use in ELISA, WB, IP.

Product Specifications

Background

The stat proteins (Stat1-6) function both as cytoplasmic signal transducers and as activators of transcription in response to cytokines and growth factor receptors. Stat3 is expressed as two variants, Stat3α (86 kDa) and Stat3b (79 kDa) that can differ in expression and activity depending on cell type, activation pathway, and cell maturation stage. Both are activated by phosphorylation at Tyr-705, which induces dimerization, nuclear translocation and DNA binding. Stat3α (86 kDa) transcriptional activation may be regulated by phosphorylation at Ser-727 through the MAPK pathway, while Stat3β lacks this serine site.

Short Description

Our Stat3 (N-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Stat3 (N-terminal region) and is Protein A purified. It is great for use in WB, IP.

Synonyms

Signal transducer and activator of transcription 3

Gene Name

STAT3

UniProt

P40763

Reactivity

Human, Mouse, and Rat

Immunogen

Clone (M263) was generated from a recombinant protein that included amino acid residues in the N-terminal region of human Stat3. This sequence has high homology to the conserved regions in rat and mouse Stat3 α and β.

Target

Stat3 (N-terminal region)

Clonality

Monoclonal

Clone

M263

Conjugation

Unconjugated

Applications

ELISA, WB, IP

Field of Research

Cell Signaling, Oncoproteins, Transcription Regulation

Format

Protein A Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Molecular Weight

79/86

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein A purified culture supernatant.

WB Dilution

1:1000

IP Dilution

1:100

Specificity

The antibody detects 86 and 79 kDa* bands corresponding to Stat3 isoforms on SDS-PAGE immunoblots of human A431 cells.

Quality Control

Western blots performed on each lot.

Host or Source

Mouse

Physical Properties

Liquid

Isotype

IgG1

Immunogen Species

Human

WB Quality Control

Quality Control: WB

RRID

AB_2198616

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