Anti-c-Src (Tyr-530) [conserved site], Phosphospecific Antibody

Our c-Src (Tyr-530) mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat c-Src (Tyr-530) and is protein a purified. It is great for use in ELISA, WB.

Product Specifications

Background

C-Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. The Src family is composed of nine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an N-terminal cell membrane anchor followed by SH3 and SH2 domains. The activity of c-Src is regulated by tyrosine phosphorylation at multiple sites. Tyrosine 418 is autophosphorylated following c-Src activation. Tyrosine 215 in the SH2 domain of c-Src is phosphorylated following growth factor receptor activation. Both Tyr-215 and Tyr-418 phosphorylation increases tyrosine kinase activity, while phosphorylation of Tyr-530 downregulates c-Src kinase activity. Thus, tyrosine phosphorylation of c-Src is critical for regulating its kinase activity.

Short Description

Our c-Src (Tyr-530) mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat c-Src (Tyr-530) and is Protein A purified. It is great for use in WB.

Synonyms

Src

Gene Name

SRC

UniProt

P12931

Modification Site

Tyr-530

Reactivity

Human, Mouse, and Rat

Immunogen

Clone (M261) was generated from a phosphopeptide containing amino acid surrounding Tyr-530 at the C-terminus of human c-Src. This sequence has high homology to the conserved site in rat and mouse c-Src, and is conserved in the c-Src family of kinases.

Target

C-Src (Tyr-530)

Clonality

Monoclonal

Clone

M261

Conjugation

Unconjugated

Applications

ELISA, WB

Field of Research

Cell Signaling, Kinase Signaling, Oncoproteins

Format

Protein A Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylated

Molecular Weight

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein A purified culture supernatant.

WB Dilution

1:500

Specificity

This antibody detects a 60 kDa* protein corresponding to c-Src on SDS-PAGE immunoblots of mouse SYF cells transformed with c-Src and treated with pervanadate. This antibody also detects the conserved site in Lck (Tyr-505) in human Jurkat cells treated with pervanadate. This antibody may detect several c-Src family members phosphorylated at the conserved site for Tyr-530.