Anti-Sox2 (Lys-119), methyl-specific Antibody

Our Sox2 (Lys-119) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and xenopus Sox2 (Lys-119) and is antigen affinity purified. It is great for use in ELISA, WB, ICC.

Product Specifications

Background

Embryonic stem cells can maintain a pluripotent state that is controlled by a set of transcription factors that include Oct-4, Sox2, and Nanog. Chromatin immunoprecipitation experiments show that Sox2 and Oct-4 bind to thousands of gene regulatory sites, many of which regulate cell pluripotency and early embryonic development. siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency, while overexpression of Oct-4 and Sox2, along with additional transcription factors Klf4 and c-Myc, can reprogram somatic cells to a pluripotent state. Sox2 also regulates adult multipotent progenitors in various epithelial tissues, and may be important for survival and regeneration of these tissues. The activity of Sox2 may be regulated by phosphorylation and methylation. Akt1 phosphorylates Thr-118 and enhances Sox2 transcriptional activity, while Set7 can monomethylate Lys-119 leading to inhibition of Sox2 transcriptional activity, as well as Sox2 ubiquination and degradation. In addition, Sox2 Thr-128 is constituitively phoshorylated in the F9 mouse stem cell line.

Short Description

Our Sox2 (Lys-119) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Sox2 (Lys-119) and is antigen affinity purified. It is great for use in WB, ICC.

Synonyms

SRY (sex determining region Y) box-2, ANOP3, MCOPS3, SOX-2

Gene Name

SOX2

UniProt

P48432

Modification Site

Lys-119

Reactivity

Chicken, Human, Mouse, Rat, and Xenopus

Immunogen

Methylated-Sox2 (Lys-119) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Lys-119 in mouse Sox2. This site is well conserved in rat and human Sox2, and has high homology with the conserved site in Sox1 (Lys-127) and Sox3 (Lys-145), but low homology to other Sox family members.

Target

Sox2 (Lys-119)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, ICC

Field of Research

Neurogenesis and Development, Oncoproteins, Transcription Regulation

Format

Antigen Affinity Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Methylated

Molecular Weight

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Affinity purified rabbit serum

WB Dilution

1:1000

Specificity

The antibody was cross adsorbed to unmethylated Sox2 (Lys-119) peptide before affinity purification using methylated-Sox2 (Lys-119) peptide. This antibody antibody detects a 34 kDa* protein on SDS-PAGE immunoblots of mouse F9 stem cells, as well as human Sox2 recombinant protein that was methylated by Set7 methyltransferase.