Anti-Slingshot-1L (C-terminal region) Antibody

Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cell motility. Regulation of cofilin activity can occur through serine phosphorylation and dephosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, Slingshot, PP1, PP2A, PP2B, and chronophin can dephosphorylate Ser-3 and activate actin binding. In mammals, the Slingshot family includes SSH1L, SSH2L, and SSH3L. SSH1L and SSH2L mRNAs are widely expressed, while SSH3L has high expression in epithelial tissues. SSH1L can associate with F-actin and may be the major phosphatase regulating cofilin activity. Disruption of SSH1L expression using RNA interference impairs directional cell migration. Thus, Slingshot phosphatases may be critical for regulating cytoskeletal protein activity and cell motility.

Our Slingshot-1L (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Slingshot-1L (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC, IHC.

A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human SSH1L. This sequence has 100% homology with similar regions of mouse and bovine SSH1L, and has low homology to SSH2L and SSH3L.

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.