Anti-Semaphorin-3E (N-terminal region) Antibody

The Semaphorin family of axon guidance molecules includes secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Semaphorin 3E (Sema-3E) is a class III secreted semaphorin that binds Plexin D1 receptor, and has putative roles in axon guidance, angiogenesis, and cancers. Mutations in the Sema-3E gene are associated with CHARGE syndrome, a disorder that has nerve and cardiovascular abnormalities. The Sema-3E protein is expressed as full length (87 kDa) and furin-dependent processed forms (61 and 25 kDa) . These forms are observed as monomers, as well as homo- and hetero-dimers. Sema-3E protein is frequently expressed in human cancer cell lines and in solid tumors from breast cancer patients. The expression of Sema-3E in mammary adenocarcinoma cells induces the ability to form experimental lung metastasis, and this activity requires furin-dependent production of the 61 kDa Sema-3E protein.

Our Semaphorin-3E (N-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Semaphorin-3E (N-terminal region) and is antigen affinity purified. It is great for use in WB.

Sema-3E (N-terminal region) synthetic peptide (coupled to carrier protein) corresponds to amino acids in the N-terminal region of human Sema-3E. This sequence is well conserved in rat, mouse, and chicken Sema-3E, and has low homology to other semaphorin family members.

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.