Anti-Phosphotyrosine Antibody

Our Anti-Phosphotyrosine phosphospecific primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, and rat Anti-Phosphotyrosine and is protein a purified. It is great for use in ELISA, WB, ICC, IP.

Product Specifications

Background

Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphotyrosine-containing proteins. Immunoprecipitation of proteins of interest, followed by detection of phosphotyrosine using anti-phosphotyrosine antibody is commonly used to correlate changes in tyrosine phosphorylation state with alterations in protein activity.

Short Description

Our Anti-Phosphotyrosine phosphospecific primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, and rat Anti-Phosphotyrosine and is Protein A purified. It is great for use in WB, ICC, IP.

Synonyms

Phosphotyrosine mAb

Modification Site

P-Tyr

Reactivity

Chicken, Human, Mouse, and Rat

Immunogen

Clone M375 was generated from tyrosine phosphorylated proteins purified from pervanadate treated A431 cells.

Target

Anti-Phosphotyrosine

Clonality

Monoclonal

Clone

M375

Conjugation

Unconjugated

Applications

ELISA, WB, ICC, IP

Field of Research

Cell Signaling, Kinase Signaling

Format

Protein A Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylated

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein A purified culture supernatant.

WB Dilution

1:1000

IP Dilution

1:100

Specificity

This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells.