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Anti-Phosphoserine/threonine Antibody

Our Anti-Phosphoserine/threonine mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Anti-Phosphoserine/threonine and is protein a purified. It is great for use in ELISA, WB, ICC, IP.

Product Specifications

Background

Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity.

Short Description

Our Anti-Phosphoserine/threonine mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Anti-Phosphoserine/threonine and is Protein A purified. It is great for use in WB, ICC, IP.

Synonyms

Phosphoser/thr mAb

Modification Site

PSer/Thr

Reactivity

Human, Mouse, and Rat

Immunogen

PM3801 Phosphoserine/threonine is a mix of two clones: Clone M380A was generated from a phosphothreonine synthetic peptide (coupled to carrier protein) and Clone M380B was generated.from a phosphoserine synthetic peptide (coupled to carrier protein) .

Target

Anti-Phosphoserine/threonine

Clonality

Monoclonal

Clone

M380A/M380B

Conjugation

Unconjugated

Applications

ELISA, WB, ICC, IP

Field of Research

Cell Signaling, Kinase Signaling

Format

Protein A Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylated

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein A purified culture supernatant.

WB Dilution

1:500

IP Dilution

1:50

Specificity

The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA.

ICC Dilution

1:50

Quality Control

Western blots performed on each lot.

Host or Source

Mouse

Physical Properties

Liquid

Isotype

IgG1

Immunogen Species

Human

WB Quality Control

Quality Control: WB

RRID

AB_1944434

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