Anti-Paxillin Antibody

Our Paxillin primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, and rat Paxillin and is protein a purified. It is great for use in ELISA, WB, ICC, IP.

Product Specifications

Background

Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during cell migration

Short Description

Our Paxillin primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, and rat Paxillin and is Protein A purified. It is great for use in WB, ICC, IP.

Gene Name

PXN

UniProt

P49023

Reactivity

Chicken, Human, Mouse, and Rat

Immunogen

Clone (M107) was generated from full-length recombinant human paxillin.

Target

Paxillin

Clonality

Monoclonal

Clone

M107

Conjugation

Unconjugated

Applications

ELISA, WB, ICC, IP

Field of Research

Cell Adhesion Molecules, Extracellular Matrix Proteins, Oncoproteins

Format

Protein A Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

GOmAb

Molecular Weight

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein A purified culture supernatant.

WB Dilution

1:1000

IP Dilution

1:100

Specificity

This antibody detects a 68kDa* protein corresponding to the molecular mass of paxillin on SDS-PAGE immunoblots of A431 cells. Similar results were seen in various cells and tissues from rat, mouse, and chicken origin.