Anti-Osteopontin (N-terminal region) Antibody

Our Osteopontin (N-terminal region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Osteopontin (N-terminal region) and is protein g purified. It is great for use in ELISA, WB, ICC.

Product Specifications

Background

Osteopontin (OPN) is a 34 kDa sialic acid rich member of small integrin-binding ligand N-linked glycoproteins. It is expressed in many different tissues and is post-translationally modified at multiple sites with both glycosylation and phosphorylation. The mature post-translationally modified protein is 60 kDa. OPN is involved with cell survival, proliferation, invasion, and stem like behavior. OPN can interact with CD44, bind hydroxyapatite, and activates many integrins. These interactions are important for OPN function in cell matrix formation. A higher presence of OPN has been found in a variety of cancers, leading to increased tumor growth and metastasis. In addition, OPN is involved in type I immunity through its function as a cytokine where it can enhance production of interferon-gamma and interleukin-12 and reduce production of interleukin-10.

Short Description

Our Osteopontin (N-terminal region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Osteopontin (N-terminal region) and is Protein G purified. It is great for use in WB, ICC.

Synonyms

Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SSP-1, Urinary stone protein, Uropontin, BNSP, OPN, SSP1, osteopontin

Gene Name

SPP1

UniProt

P10451

Reactivity

Human, Mouse, and Rat

Immunogen

Clone M574 was generated from a recombinant protein containing amino acid residues in the N-terminal region of human osteopontin. This sequence has high homology with rat and mouse osteopontin.

Target

Osteopontin (N-terminal region)

Clonality

Monoclonal

Clone

M574

Conjugation

Unconjugated

Applications

ELISA, WB, ICC

Field of Research

Cell Adhesion Molecules, Extracellular Matrix Proteins, Oncoproteins

Format

Protein G Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Molecular Weight

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein G purified culture supernatant.

WB Dilution

1:500

Specificity

Clone M574 was purified using Protein G chromatography. The antibody detects a 60 kDa* protein corresponding to the molecular mass of Osteopontin on SDS-PAGE immunoblots of human MDA-MB-231 cells and human full length recombinant Osteopontin protein.