Anti-eNOS (Ser-632), Phosphospecific Antibody

Our eNOS (Ser-632) mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat eNOS (Ser-632) and is protein a purified. It is great for use in ELISA, WB.

Product Specifications

Background

Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme) . Several distinct NOS isoforms are produced from three distinct genes. The inducible form of NOS, iNOS (NOS-II), is Ca2+ independent and is expressed in a broad range of cell types, and two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (bNOS, NOS-I) identified in neurons and eNOS (ecNOS, NOS-III) identified in endothelial cells. Regulation of eNOS activity occurs through phosphorylation at multiple sites. Phosphorylation of Ser-633 (mouse Ser-632) in the FMN binding domain increases eNOS activity and may be important for the maintenance of NO synthesis after initial activation by Ca2+ flux and Ser-1177 phosphorylation.

Short Description

Our eNOS (Ser-632) mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat eNOS (Ser-632) and is Protein A purified. It is great for use in WB.

Synonyms

Endothelial Nitric Oxide Synthase, eNOS, ecNOS, NOS-III, NOS3, NOSIII

Gene Name

NOS3

UniProt

P70313

Modification Site

Ser-632

Reactivity

Human, Mouse, and Rat

Immunogen

Clone M232 was generated from a Phospho-eNOS (Ser-632) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-632 in mouse eNOS. This sequence is conserved in human (Ser-633) and rat (Ser-632) eNOS, and has low homology to other NOS family members.

Target

ENOS (Ser-632)

Clonality

Monoclonal

Clone

M232

Conjugation

Unconjugated

Applications

ELISA, WB

Field of Research

Cell Signaling

Format

Protein A Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylated

Molecular Weight

140

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein A purified culture supernatant.

WB Dilution

1:500

Specificity

The antibody detects a 140 and 120 kDa* bands on SDS-PAGE immunoblots of human umbilical vein endothelial cells, but these bands are not observed after lambda phosphatase treatment. The 120 kDa band may be a truncated form of eNOS.