Anti-Na+/K+ ATPase β3 (Extracellular) Antibody

Our Na+/K+ ATPase β3 (Extracellular) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Na+/K+ ATPase β3 (Extracellular) and is protein g purified. It is great for use in ELISA, WB, ICC, IP.

Product Specifications

Background

The Na+/K+ ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving Na+ export and K+ import across the plasma membrane. It is composed of a large catalytic α subunit and a membrane-spanning auxiliary β subunit. In humans, the Na+/K+ ATPase is a binary complex of an α subunit that has four isoforms (α1-α4) and a β-subunit that has three isoforms (β1, β2, β3) . Na+/K+ ATPase subunit expression has been shown to be upregulated in cancers, and inhibition of Na+/K+ ATPase activity has anti-cancer effects. The β3 subunit of Na+/K+ ATPase has increased expression in human gastric cancer tissues and cell lines, and its increased expression level predicts poor patient outcome. β3 subunit knockdown significantly inhibited cell proliferation, colony-formation ability, migration, and invasion in human gastric carcinoma cell lines.

Short Description

Our Na+/K+ ATPase β3 (Extracellular) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Na+/K+ ATPase β3 (Extracellular) and is Protein G purified. It is great for use in WB, ICC, IP.

Synonyms

Sodium/potassium-transporting ATPase subunit beta-3, ATPB-3, CD298, ATP1B3

Gene Name

ATP1B3

UniProt

P54709

Reactivity

Human

Immunogen

Clone (M016) was generated from a proprietary antigen related to the native human Na+/K+ ATPase β3 subunit expressed in A431 epidermoid carcinoma cell line.

Target

Na+/K+ ATPase β3 (Extracellular)

Clonality

Monoclonal

Clone

M016

Conjugation

Unconjugated

Applications

ELISA, WB, ICC, IP

Field of Research

Ion Channels and Modulators, K+ Channels, Na+ Channels, Cell Signaling, Oncoproteins

Format

Protein G Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

GOmAb

Molecular Weight

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein G purified culture supernatant.

WB Dilution

1:1000

IP Dilution

1:50

Specificity

Clone M016 mouse monoclonal antibody detects a 40 kDa* protein on SDS-PAGE "Native" immunoblots of human A431, LNCaP, MeWo, MDA-MB-231, and MCF7 cells. This antibody does not detect denatured Na+/K+ ATPase β3 subunit. In addition, mass spectrometry analysis of immunoprecipitates using NM0161 in human A431 cell lysates confirms that this antibody only detects Na+/K+ ATPase β3 protein.The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry, as well as detects the β3 subunit on live cells.