Anti-Laminin β2/γ1 Subunits Antibody

Our Laminin β2/γ1 Subunits Antibody mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Laminin β2/γ1 Subunits Antibody and is protein a purified. It is great for use in ELISA, WB (Nat), ICC.

Product Specifications

Background

The human basal lamina contains Collagen Type IV, proteoglycans, and glycoproteins. Laminin is a high molecular weight (850 kDa) oligomer, consisting of three different chains laminin alpha (α), beta (β), and gamma (γ) joined by disulfide bonds. The structure of human laminins include two helical domains (I & II) at the COOH-terminal, a laminin IV domain, multiple EGF-like repeats, and a laminin globular domain (G), as well as an N-terminal domain VI. Domains IV and VI are the binding sites for collagen and heparan sulfate, respectively. Several isoforms have been identified for the genes of each chain including 5 alpha chains, 4 beta chains, and 3 gamma chains. Laminin β2 and γ1 are found in laminin 121, laminin 221, laminin 421, and laminin 521. The expression of the Laminin subunits is found in the basal lamina of tissues. Here, the protein interacts with other extracellular matrix components to mediate cell attachment, migration and organization during embryonic development.

Short Description

Our Laminin β2/γ1 Subunits Antibody mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Laminin β2/γ1 Subunits Antibody and is Protein A purified. It is great for use in WB (Nat), ICC.

Synonyms

Laminin subunit gamma-1, Laminin B2 chain Laminin-1 Laminin-10 Laminin-11 Laminin-2 Laminin-3 Laminin-4 Laminin-6 Laminin-7 Laminin-8 Laminin-9 S-laminin S-LAM gamma LAMC1 LAMB2 Laminin 521, Laminin beta 2

Gene Name

LAMC1

UniProt

P11047

Reactivity

Human

Immunogen

Clone (M046) was generated from a recombinant human laminin 521 protein that includes a heterotrimer of three subunits laminin α5, laminin β2, and laminin γ1 subunits.

Target

Laminin β2/γ1 Subunits Antibody

Clonality

Monoclonal

Clone

M046

Conjugation

Unconjugated

Applications

ELISA, WB (Nat), ICC

Field of Research

Cell Adhesion Molecules, Cell Signaling, Cytoskeletal Regulation, Extracellular Matrix Proteins, Neurogenesis and Development

Format

Protein A Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Molecular Weight

760

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein A purified culture supernatant.

WB Dilution

1:1000

Specificity

Clone M046 detects native laminin isoforms that include both laminin β2 and γ1 subunits. In native immunoblots, the antibody detects laminin 521, laminin 221, and laminin 121, but does not detect lamin 332 and laminin 411. The antibody weakly detects laminin 511. This antibody can be used for immunocytochemical detection of laminin β2/γ1 subunits, and can also be used for detection of native laminin isoforms in immunoblots and ELISA.