Anti-Integrin β1 (Extracellular region) Antibody

Our Integrin β1 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Integrin β1 (Extracellular region) and is protein g purified. It is great for use in ELISA, WB, ICC.

Product Specifications

Background

Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The integrin β2 subunit associates with integrin αL to form a receptor for ICAM family members. Integrin β2/αL is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.

Short Description

Our Integrin β1 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Integrin β1 (Extracellular region) and is Protein G purified. It is great for use in WB, ICC.

Synonyms

Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29, TGB1, FNRB, MDF2, MSK12, ITGB1

Gene Name

ITGB1

UniProt

P05556

Reactivity

Human

Immunogen

Clone M032 was generated from a proprietary antigen related to the extracellular region of human integrin β1 expressed in A431 epidermoid carcinoma cell line.

Target

Integrin β1 (Extracellular region)

Clonality

Monoclonal

Clone

M032

Conjugation

Unconjugated

Applications

ELISA, WB, ICC

Field of Research

Cell Adhesion Molecules, Cell Signaling, Cytoskeletal Regulation, Oncoproteins

Format

Protein G Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Molecular Weight

120-150

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein G purified culture supernatant.

WB Dilution

1:500

Specificity

Clone M032 detects a 120-150 kDa* band corresponding to Integrin β1 on SDS-PAGE immunoblots of "Native" human A431, A549, MDA-MB-231, and LNCaP cell lysates, as well as human breast, skin, and lung tissues. The antibody weakly detects the denatured form of Integrin β1. Clone M032 can be used in western blot, immunocytochemistry, and ELISA.