Anti-Histone H4 (Tyr-72), Phosphospecific Antibody

Our Histone H4 (Tyr-72) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and xenopus Histone H4 (Tyr-72) and is antigen affinity purified. It is great for use in ELISA, WB.

Product Specifications

Background

Chromatin structure is regulated through the activity of core histones (H2A, H2B, H3, and H4) that form the nucleosome. Histone activity is regulated by a variety of post-translational modifications, including acetylation, phosphorylation, and methylation. Histone acetylation and methylation occur primarily at lysine (K) residues in the amino-terminal tail domain. These modifications are important for the regulation of histone deposition, transcriptional activation, DNA replication and repair. Acetylation and methylation of specific lysine residues creates docking sites for DNA repair, transcription, and chromatin regulatory proteins. Methylation of histones may be regulated by phosphorylation events at sites downstream of the N-terminal tail. In histone H4, both EGFR activation and inonizing radiation induce EGFR nuclear translocation and Histone H4 (Tyr-72) phosphorylation, which creates a docking site for Set8 methyltransferase. This promotes K20 methylation in Histone H4 leading to DNA synthesis and repair.

Short Description

Our Histone H4 (Tyr-72) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Histone H4 (Tyr-72) and is antigen affinity purified. It is great for use in WB.

Synonyms

Hist1H4 Histone H4

Gene Name

H4C1

UniProt

P62805

Modification Site

Tyr-72

Reactivity

Chicken, Human, Mouse, Rat, and Xenopus

Immunogen

Phospho-Histone H4 (Tyr-72) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Tyr-72 in human histone H4. This site is well conserved in rat and mouse histone H4, but the site is not found in other histone family members.

Target

Histone H4 (Tyr-72)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Field of Research

DNA Damage and Repair, Epigenetics, Oncoproteins, Transcription Regulation

Format

Antigen Affinity Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylated

Molecular Weight

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Affinity purified rabbit serum

WB Dilution

1:1000

Specificity

The antibody was cross adsorbed to unphosphorylated histone H4 (Tyr-72) peptide before affinity purification using phospho-histone H4 (Tyr-72) peptide. This antibody antibody detects a 10 kDa* protein on SDS-PAGE immunoblots of human PC3 and A431 cells. This reactivity is not observed after alkaline phosphatase dephosphorylation of histone H4.