Anti-Girdin (Ser-1416), Phosphospecific Antibody

Our Girdin (Ser-1416) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Girdin (Ser-1416) and is antigen affinity purified. It is great for use in ELISA, WB.

Product Specifications

Background

Girdin, a member of the CCDC88 (Hook related protein) family, is an actin binding protein involved with cell migration and maintaining cytoskeletal organization. Girdin has conserved domains at the N- and C-terminus that bind microtubules and actin, respectively. It enhances PI3-kinase dependent phosphorylation of proteins, most notably Akt. This same activity can contribute to tumor proliferation, invasion, and metastasis in breast, ovarian, prostate, and pancreatic tissues. Girdin is phosphorylated at three separate locations: Ser-1416, Ser-1674, and Tyr-1764. Ser-1416 is the primary Akt phosphorylation site, while Cyclin-dependent kinases interact with Girdin and phosphorylate Ser-1674. Multiple receptor tyrosine kinases can bind girdin and phosphorylate Tyr-1764.

Short Description

Synonyms

APE, Galpha, vesicle, GIV, Girders actin filament, HkRP1, GRDN, CCDC88A

Gene Name

CCDC88A

UniProt

Q3V6T2

Modification Site

Ser-1416

Reactivity

Human, Mouse, and Rat

Immunogen

Phospho-Girdin (Ser-1416) synthetic peptide (coupled to carrier) corresponding to amino acid residues around serine 1416 in human Girdin. This sequence is identical in rat and mouse Girdin, and has low homology to similar regions in other Girdin family members.

Target

Girdin (Ser-1416)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Field of Research

Cell Adhesion Molecules, Cell Signaling, Cytoskeletal Regulation, Neurogenesis and Development, Oncoproteins

Format

Antigen Affinity Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylated

Molecular Weight

250

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Affinity purified rabbit serum

WB Dilution

1:500

Specificity

The antibody was cross-adsorbed to unphosphorylated Girdin (Ser-1416) peptide before affinity purification using phospho-Girdin (Ser-1416) peptide. This antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of human A431 cells stimulated with EGF or calyculin A. These reactivities are not observed after lambda phosphatase treatment to dephosphorylate Girdin.