Anti-Girdin (C-terminus) Antibody

Our Girdin (C-terminus) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Girdin (C-terminus) and is protein g purified. It is great for use in ELISA, WB, ICC, IHC, IP.

Product Specifications

Background

Girdin, a member of the CCDC88 (Hook related protein) family, is an actin binding protein involved with cell migration and maintaining cytoskeletal organization. Girdin has conserved domains at the N- and C-terminus that bind microtubules and actin, respectively. It enhances PI3-kinase dependent phosphorylation of proteins, most notably Akt. This same activity can contribute to tumor proliferation, invasion, and metastasis in breast, ovarian, prostate, and pancreatic tissues. Girdin is phosphorylated at three separate locations: Ser-1416, Ser-1674, and Tyr-1764. Ser-1416 is the primary Akt phosphorylation site, while Cyclin-dependent kinases interact with Girdin and phosphorylate Ser-1674. Multiple receptor tyrosine kinases can bind girdin and phosphorylate Tyr-1764.

Short Description

Our Girdin (C-terminus) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Girdin (C-terminus) and is Protein G purified. It is great for use in WB, ICC, IHC, IP.

Synonyms

APE, Galpha, vesicle, GIV, Girders actin filament, HkRP1, GRDN, CCDC88A

Gene Name

CCDC88A

UniProt

Q3V6T2

Reactivity

Human

Immunogen

Clone (M012) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human Girdin.

Target

Girdin (C-terminus)

Clonality

Monoclonal

Clone

M012

Conjugation

Unconjugated

Applications

ELISA, WB, ICC, IHC, IP

Field of Research

Cell Adhesion Molecules, Cell Signaling, Cytoskeletal Regulation, Neurogenesis and Development, Oncoproteins

Format

Protein G Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Molecular Weight

250

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein G purified culture supernatant.

IHC Dilution

1:50

WB Dilution

1:250

IP Dilution

1:50

Specificity

Clone M012 mouse monoclonal antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of human A431 cells and human brain tissue. The antibody also works for immmunoprecipitation, immunohistochemistry, and immunocytochemistry.