Anti-Fibronectin (Cell/Heparin2 Binding region) Antibody

Our Fibronectin (Cell/Heparin2 Binding region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Fibronectin (Cell/Heparin2 Binding region) and is protein g purified. It is great for use in ELISA, WB, ICC, IP.

Product Specifications

Background

In the extracellular matrix, fibronectin provides essential connections to cells through interaction with integrins and other receptors that regulate cell adhesion, migration, and differentiation. Fibronectin is secreted as a large dimeric glycoprotein with subunits that range in size from 230 kDa to 270 kDa. Fibronectin is composed of three different types of modules termed type I, II, and III repeats, as well as two fibrin binding and two heparin binding domains, a collagen interaction region and cell attachment domain. The diverse set of binding domains provides fibronectin with the ability to interact simultaneously with other fibronectin molecules, other ECM components (e.g., collagens and proteoglycans), cell surface receptors, and extracellular enzymes. Plasma fibronectin (soluble dimeric form) is secreted by hepatocytes, while cellular fibronectin (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Fibronectin fibrilogenesis has important functions during tissue development, and during pathological progression of tissues and wound healing.

Short Description

Our Fibronectin (Cell/Heparin2 Binding region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Fibronectin (Cell/Heparin2 Binding region) and is Protein G purified. It is great for use in WB, ICC, IP.

Synonyms

FN, Cold-insoluble globulin, CIG, Anastellin, Ugl-Y1, Ugl-Y2, Ugl-Y3, FN1

Gene Name

FN1

UniProt

P02751

Reactivity

Human

Immunogen

Clone (M013) was generated from a proprietary antigen related to native human fibronectin from the A549 lung carcinoma cell line.

Target

Fibronectin (Cell/Heparin2 Binding region)

Clonality

Monoclonal

Clone

M013

Conjugation

Unconjugated

Applications

ELISA, WB, ICC, IP

Field of Research

Cell Adhesion Molecules, Extracellular Matrix Proteins, Neurogenesis and Development

Format

Protein G Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

GOmAb

Molecular Weight

250

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein G purified culture supernatant.

WB Dilution

1:1000

IP Dilution

1:50

Specificity

Clone M013 mouse monoclonal antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of native human A549, MeWo, and MDA-MB-231 cells, as well as human plasma fibronectin. The antibody does not detect the denatured form of fibronectin. The antibody also works for immunoprecipitation, immunohistochemistry, and immunocytochemistry, as well as binds fibronectin in live cells and unfixed extracellular matrix.