Anti-Fascin (Ser-39), Phosphospecific Antibody

Our Fascin (Ser-39) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Fascin (Ser-39) and is antigen affinity purified. It is great for use in ELISA, WB, ICC.

Product Specifications

Background

Fascin is an actin filament bundling protein localized to lamellipodia and filopodia where it has important roles in cell motility. Regulation of fascin occurs through PKC-mediated phosphorylation of Ser-39 in the F-actin binding site. Cell permeant peptides that block PKC phosphorylation of Ser-39 increase cell migration, while peptides that block fascin binding to F-actin alter lamellipodial morphology and cause aberrant cell motility. Studies using RNA interference of fascin show that fibroblasts have reduced number and abnormal morphology of filopodia, while Ser-39 phosphorylation status may determine filopodial frequency. In Drosophila neurons, fascin deficiency causes alterations in actin filaments and leads to abnormal morphology of developing neurons. Thus, fascin is a critical element of actin-based motility in various cell types.

Short Description

Synonyms

P55

Gene Name

FSCN1

UniProt

Q16658

Modification Site

Ser-39

Reactivity

Chicken, Human, Mouse, and Rat

Immunogen

Fascin (Ser-39) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding serine 39 in human fascin. This sequence has high homology to the conserved site in rat and mouse fascin, as well as to the conserved region in fascin-2, but has low homology to the conserved region in fascin-3.

Target

Fascin (Ser-39)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, ICC

Field of Research

Cell Signaling, Cytoskeletal Regulation

Format

Antigen Affinity Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylated

Molecular Weight

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Affinity purified rabbit serum

WB Dilution

1:1000

Specificity

This antibody was cross-adsorbed to a phospho-serine peptide before affinity purified using phosho-fascin (Ser-39) peptide (without carrier) . The antibody detects a 55 kDa* band corresponding to fascin on SDS-PAGE immunoblots of mouse C2C12 and human HeLa cells treated with Calyculin A. This band is not observed after lambda phosphatase treatment. The antibody also detects fascin (Ser-39) phosphorylation in chick DRG neurons.