Anti-FAK (Tyr-397), Phosphospecific Antibody

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in signal transduction pathways important for cell spreading, migration and survival. Activation of FAK by integrin clustering leads to autophosphorylation at Tyr-397, which is a binding site for Src family kinases, PI3-Kinase, and PLCγ. The recruitment of Src family kinases results in the phosphorylation of tyrosine 407, 576, and 577 in the catalytic domain, and tyrosine 871 and 925 in the carboxy-terminal region of FAK. Thus, the phosphorylation of Tyr-397 is a critical step in the activation of FAK.

Our FAK (Tyr-397) mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat, rabbit FAK (Tyr-397) and is Protein A purified. It is great for use in WB.

Clone (M121) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 397 of human FAK. This peptide sequence has high homology to the conserved tyrosine site in rat and mouse FAK.

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

The antibody detects a 125 kDa* protein on SDS-PAGE immunoblots of untreated HUVEC cells. This phosphorylated band is greatly reduced after treatment with alkaline phosphatase.