Anti-Estrogen Receptor α (Tyr-537), Phosphospecific Antibody

Our Estrogen Receptor α (Tyr-537) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and xenopus Estrogen Receptor α (Tyr-537) and is antigen affinity purified. It is great for use in ELISA, WB.

Product Specifications

Background

Estrogen receptor α (ERα) is a member of the steroid receptor superfamily and its structure includes an N-terminal ligand-independent transactivation domain (AF-1), a highly conserved DNA binding domain, and a C-terminal ligand-dependent transactivation domain (AF-2) . AF-1 and AF-2 activate transcription independently and synergistically, and act in a promoter- and cell-specific manner. Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity. Ser-104, Ser-106, Ser-118, and Ser-167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. In addition to these sites, phosphorylation of Tyr-537 has been implicated in maximal hormone binding, dimerization, and transcriptional activity. Tyr-537, located in the AF-2 domain, is phosphorylated by c-Src leading to nuclear export of ERα and degradation. Thus, a variety of phosphorylation events control ERα activity.

Short Description

Our Estrogen Receptor α (Tyr-537) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Estrogen Receptor α (Tyr-537) and is antigen affinity purified. It is great for use in WB.

Synonyms

ESR, ESR1, ESRA, Estradiol receptor, Eralpha, ER

Gene Name

ESR1

UniProt

P03372

Modification Site

Tyr-537

Reactivity

Chicken, Human, Mouse, Rat, and Xenopus

Immunogen

Phospho-ERα (Tyr-537) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Tyr-537 in human ERα. This sequence is well conserved in rat and mouse ERα, and is also well conserved in ERβ (Tyr-488) .

Target

Estrogen Receptor α (Tyr-537)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Field of Research

Nuclear Hormone Receptors, Cell Signaling, Oncoproteins

Format

Antigen Affinity Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylated

Molecular Weight

35-66

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Affinity purified rabbit serum

WB Dilution

1:1000

Specificity

The antibody was cross-adsorbed to unphosphorylated ERα (Tyr-537) peptide before affinity purification using phospho-ERα (Tyr-537) peptide (without carrier) . This antibody detects several forms of ERα ranging from 66 to 35 kDa* on SDS-PAGE immunoblots of MCF-7 cells treated with pervanadate, and this reactivity is removed after alkaline phosphatase treatment.