Anti-EphA2 (Extracellular region) M060 Antibody

Our EphA2 (Extracellular region) M060 Antibody mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human EphA2 (Extracellular region) M060 Antibody and is protein g purified. It is great for use in ELISA, WB (Nat), ICC.

Product Specifications

Background

The Eph family of receptor tyrosine kinases and their Ephrin ligands are important for cell positioning and morphogenesis during development. Eph receptors are classified into 10 EphA and 6 EphB receptors, which preferentially bind to the type A and type B ephrins, respectively. Ephrin type-A receptor 2 (EphA2), also known as epithelial cell kinase (Eck), binds the ephrin A1 (EFNA1) ligand, and has roles in neuronal development and repair, as well as carcinogenesis. EphA2 receptor has an N-terminal ligand-binding domain followed by a cysteine-rich domain with an epidermal growth factor-like motif and two fibronectin type-III repeats in the extracellular region, and a sterile alpha motif (SAM), and a PDZ domain-binding motif in the intracellular region. EphA2 is expressed in many types of cancers, including breast, colon, bladder, gastric, and glioblastoma. In bladder cancers, EphA2 may be activated by progranulin leading to phosphorylation at Ser-897 and bladder tumorigenesis. EphA2 may be an important therapeutic target and biomarker for several types of cancer.

Short Description

Our EphA2 (Extracellular region) M060 Antibody mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human EphA2 (Extracellular region) M060 Antibody and is Protein G purified. It is great for use in WB (Nat), ICC.

Synonyms

EphA2, P29317, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK, Ephrin type-A receptor 2

Gene Name

EPHA2

UniProt

P29317

Reactivity

Human

Immunogen

Clone (M060) was generated from a recombinant protein that included the extracellular region of human EphA2 protein.

Target

EphA2 (Extracellular region) M060 Antibody

Clonality

Monoclonal

Clone

M060

Conjugation

Unconjugated

Applications

ELISA, WB (Nat), ICC

Field of Research

Cardiovascular, Cell Signaling, Kinase Signaling, Oncoproteins

Format

Protein G Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

GOmAb

Molecular Weight

125

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein G purified culture supernatant.

WB Dilution

1:1000

Specificity

Clone M060 mouse monoclonal antibody detects a 125kDa* protein on SDS-PAGE "Native" immunoblots of human NCI-H2052 and MDA-MB-231 carcinomas, as well as the EphA2 C-terminal 60 kDa fragement in human A431 and A549. The antibody detects native EphA2, as well as aldehyde-fixed EphA2. The antibody works for Native western blot, ELISA capture, and immunocytochemistry.