Anti-EpCAM (Extracellular region) Antibody

Our EpCAM (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human EpCAM (Extracellular region) and is protein g purified. It is great for use in ELISA, WB, ICC, IP.

Product Specifications

Background

Epithelial Cell Adhesion Molecule (EpCAM) is a signal type I transmembrane glycoprotein that has an extracellular domain with one thyroglobulin type-1 domain and a short cytoplasmic domain. EpCAM is found on the surface of adenocarcinoma, but not on mesodermal or neural cell membranes. The EpCAM molecule has been shown to function as a homophilic Ca2+ independent adhesion molecule. It may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium. Defects in EpCAM cause hereditary non-polyposis colorectal cancer type 8 (HNPCC8) and diarrhea type 5 (DIAR5) . EpCAM plays a role in embryonic stem cell proliferation and differentiation; it up-regulates the expression of FABP5, MYC, and Cyclin A & Cyclin E. It is highly and selectively expressed by undifferentiated embryonic stem cells and in many types of epithelial carcinomas.

Short Description

Our EpCAM (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human EpCAM (Extracellular region) and is Protein G purified. It is great for use in WB, ICC, IP.

Synonyms

GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, Epitelial glycoprotein, EGP, KS 1/4 antigen, KSA, EGP314, CD326, EpCAM

Gene Name

EPCAM

UniProt

P16422

Reactivity

Human

Immunogen

Clone (M042) was generated from the extracellular region of human EpCAM.

Target

EpCAM (Extracellular region)

Clonality

Monoclonal

Clone

M042

Conjugation

Unconjugated

Applications

ELISA, WB, ICC, IP

Field of Research

Cell Adhesion Molecules, Oncoproteins

Format

Protein G Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

GOmAb

Molecular Weight

35-45

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein G purified culture supernatant.

WB Dilution

1:500

IP Dilution

1:50

Specificity

Clone M042 mouse monoclonal antibody detects a 35-45 kDa* protein on SDS-PAGE "Native" immunoblots of human A431, H1915, and MCF7 carcinomas. This antibody does not detect denatured EpCAM. The antibody works for native western blot, immunoprecipitation, ELISA, and immunocytochemistry.