Anti-EGFR (Ser-967), Phosphospecific Antibody

Our EGFR (Ser-967) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat EGFR (Ser-967) and is antigen affinity purified. It is great for use in ELISA, WB, ICC.

Product Specifications

Background

The epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein with an extracellular ligand-binding domain and a cytoplasmic domain with intrinsic tyrosine kinase activity. The cytoplasmic domain has a C-terminal region with multiple autophosphorylation sites (Tyr-992, 1068, 1086, 1148, and 1173) . These sites are important for downstream signaling and rapid internalization. In addition, EGFR activation leads to c-Src mediated phosphorylation of Tyr-845 and Tyr-1101. The former site is required for mitogenic responses to EGFR activation, while the latter may be an SH2 binding site. Phosphorylation of EGFR on serine and threonine residues is thought to represent a mechanism for regulation of receptor kinase activity and internalization. These sites include a PKC site (Thr-654), CAMKII sites (Ser-1046, 1047, 1057, and 1142), and constitutively phosphorylated sites (Ser-967 and Ser-1002) . Thus, the regulation of EGFR activity involves a complex series of phosphorylation events at multiple sites throughout the intracellular portion of the receptor.

Short Description

Synonyms

EGF, Epidermal, ErbB

Gene Name

EGFR

UniProt

P00533

Modification Site

Ser-967

Reactivity

Human, Mouse, and Rat

Immunogen

Phospho-EGFR (Ser-967) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 967 of human EGFR (ErbB-1) . This human EGFR sequence has high homology to rat and mouse EGFR, and low homology to other ErbB family members.

Target

EGFR (Ser-967)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, ICC

Field of Research

Cell Adhesion Molecules, Cell Signaling, Growth Factor Signaling, Kinase Signaling, Oncoproteins

Format

Antigen Affinity Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylated

Molecular Weight

180

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Affinity purified rabbit serum

WB Dilution

1:1000

Specificity

This antibody was cross-adsorbed to unphosphorylated EGFR (Ser-967) coupled to agarose then affinity purified using phospho-EGFR (Ser-967) peptide (without carrier) . The antibody detects a 180 kDa* protein on SDS-PAGE immunoblots of human A431 cells treated with Calyculin A, and does not detect this band after lambda phosphatase treatment.