Anti-eEF2K (Ser-445), Phosphospecific Antibody

Our eEF2K (Ser-445) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, rat, and xenopus eEF2K (Ser-445) and is antigen affinity purified. It is great for use in ELISA, WB.

Product Specifications

Background

Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. eEF2 kinase (eEF2K) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation. eEF2K is normally dependent on Ca2+ ions and calmodulin, and can be activated by PKA in response to elevated cAMP levels during cell stress- or starvation-related conditions. Regulation of eEF2K occurs through phosphorylation at multiple sites. Ser-78 phosphorylation is required for calmodulin binding and eEF2K activity, while phosphorylation of Ser-500 is required for Ca2+/calmodulin-independent kinase activity. Thr-348 is an autophosphorylation site that is required for kinase activity. Inhibitory phosphorylation may also regulate eEF2K, since phosphorylation at Ser-359 by SAPK4/p38δ causes inactivation of eEF2K. Thus, multisite phospho-regulation of eEF2K may be important for proper control of eEF2K activity and protein translation.

Short Description

Our eEF2K (Ser-445) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, rat, and Xenopus eEF2K (Ser-445) and is antigen affinity purified. It is great for use in WB.

Synonyms

EEF-2, eEF-2K, CaMK-III, eukaryotic elongation factor

Gene Name

EEF2K

UniProt

O00418

Modification Site

Ser-445

Reactivity

Human, Mouse, Rat, and Xenopus

Immunogen

Phospho-eEF2K (Ser-445) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-445 in human eEF2K. This sequence is well conserved in rat, mouse, and fish eEF2K, and has low homology to other proteins.

Target

EEF2K (Ser-445)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Field of Research

Apoptosis, Cell Signaling, Kinase Signaling, Epigenetics, Oncoproteins

Format

Antigen Affinity Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylated

Molecular Weight

105

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Affinity purified rabbit serum

WB Dilution

1:1000

Specificity

The antibody was cross-absorbed to unphosphorylated eEF2K (Ser-445) before affinity purification using phospho-eEF2K (Ser-445) peptide (without carrier) . This antibody detects a 105 kDa* protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of Jurkat cells treated with Calyculin A, and this reactivity is removed after lambda phosphatase treatment.