Anti-Early Endosome Antigen 1 (EEA1) Antibody

Our Early Endosome Antigen 1 (EEA1) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Early Endosome Antigen 1 (EEA1) and is protein a purified. It is great for use in ELISA, WB, ICC.

Product Specifications

Background

Early endosomes are cellular compartments that receive endocytosed materials and sort them for vesicular transport to late endosomes and lysosomes, as well as for recycling material to the plasma membrane. Early endosome antigen 1 (EEA1) is an early endosomal protein that contains an N-terminal zinc finger motif, a cys-rich C-terminal metal-binding finger, and multiple sites for N-glycosylation, phosphorylation, and N-myristoylation. Expression of EEA1 mRNA is observed in skeletal muscle, heart, brain, lung, liver, and pancreas. Immunoblot analysis shows that EEA1 is a 180-kDa protein localized in membrane and cytosolic fractions. Immunofluorescence microscopy shows that EEA1 colocalizes with transferrin and with RAB5 in early endosomes, but not with RAB7 in late endosomes.

Short Description

Our Early Endosome Antigen 1 (EEA1) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Early Endosome Antigen 1 (EEA1) and is Protein A purified. It is great for use in WB, ICC.

Gene Name

EEA1

UniProt

Q15075

Reactivity

Human, Mouse, and Rat

Immunogen

Clone M347 was generated from a recombinant protein corresponding to amino acid residues in the N-terminal region of human EEA1. This sequence has high homology to similar regions in rat and mouse EEA1.

Target

Early Endosome Antigen 1 (EEA1)

Clonality

Monoclonal

Clone

M347

Conjugation

Unconjugated

Applications

ELISA, WB, ICC

Field of Research

Cell Markers, Neuronal Markers, Vesicle Trafficking/Fusion

Format

Protein A Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

GOmAb

Molecular Weight

180

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Protein A purified culture supernatant.

WB Dilution

1:1000

Specificity

This antibody detects a 180 kDa* protein corresponding to the apparent molecular mass of EEA1 on SDS-PAGE immunoblots of adult mouse brain. In immunocytochemistry, anti-EEA1 specifically stains endosomal vesicles.