Anti-mDia2 (Thr-1061), Phosphospecific Antibody

Our mDia2 (Thr-1061) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and xenopus mDia2 (Thr-1061) and is antigen affinity purified. It is great for use in ELISA, WB.

Product Specifications

Background

Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. The FH1 region contains poly-proline stretches that promote interactions with profilin. The FH2 domain, located C-terminally to the FH1 domain, is highly conserved in formin proteins and possesses actin nucleation and polymerization activities. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. A subgroup of the formins is the diaphanous (Dia) family, which includes mDia1 (Diap1), mDia2 (Diap3), and mDia3 (Diap2) . The mDia2 protein has been implicated in cell migration and cytokinesis. This Dia protein can nucleate actin polymerization, as well as bind and stabilize microtubules. mDia2 may also have functions in the nucleus, since it is continually shuttled between the cytoplasm and nucleus

Short Description

Our mDia2 (Thr-1061) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus mDia2 (Thr-1061) and is antigen affinity purified. It is great for use in WB.

Synonyms

Diap3, Dia2, Drf3, formin

Gene Name

DIAPH2

UniProt

Q9NSV4

Modification Site

Thr-1061

Reactivity

Chicken, Human, Mouse, Rat, and Xenopus

Immunogen

Phospho-mDia2 (Thr-1061) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Thr-1061 in mouse mDia2 (Diap3) . This peptide sequence is identical in rat and has 2 amino acid differences to human mDia2. The sequence has low homology to other formins.

Target

MDia2 (Thr-1061)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB

Field of Research

Cell Signaling, Cytoskeletal Regulation, Oncoproteins

Format

Antigen Affinity Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylated

Molecular Weight

135

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Affinity purified rabbit serum

WB Dilution

1:1000

Specificity

The antibody detects a 135 kDa* protein corresponding to mDia2 in immunoblots of human A7r5 and mouse C2C12 cells treated with calyculin A. The antibody detects transfected mDia2 after treatment with calyculin A, but this reactivity is removed after phosphatase treatment. In addition, the antibody does not detect mDia2 (T1061A) mutant.