Anti-CXCR4 (Ser-324/Ser-325), Phosphospecific Antibody

Our CXCR4 (Ser-324/Ser-325) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat CXCR4 (Ser-324/Ser-325) and is antigen affinity purified. It is great for use in ELISA, WB, ICC, IHC.

Product Specifications

Background

The chemokine receptor CXCR4 is a widely expressed G protein-coupled receptor required for development, hematopoiesis, organogenesis, and vascularization. In disease, CXCR4 has been implicated in WHIM syndrome, HIV, and cancers. Regulation of CXCR4 function occurs through phosphorylation at multiple sites in the C-terminal region. These sites have been shown to be phosphorylated after CXCL12 stimulation, and involve several kinases, such as PKC and GRK kinases. After CXCL12 stimulation of HEK293 cells, Ser-324 and Ser-325 become phosphorylated by PKC and GRK6, while Ser-330 and Ser-339 are phosphorylated by only GRK6. In human astroglia cells, Ser-324 and Ser-325 are rapidly phosphorylated in endogenous CXCR4, while Ser-330 was phosphorylated with slower kinetics. In addition, arrestin binding to CXCR4 is driven by this phosphorylation of far C-terminal residues. Thus, site-specific phosphorylation of CXCR4 may be regulated by multiple kinases lead to complex regulation of CXCR4 signaling.

Short Description

Synonyms

FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E; CXCR4

Gene Name

CXCR4

UniProt

P61073

Modification Site

S324/S325

Reactivity

Chicken, Human, Mouse, and Rat

Immunogen

Phospho-CXCR4 (Ser-324/Ser-325) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-324 and Ser-325 in the C-terminal region of human CXCR4. This sequence is conserved in mouse, rat, and chicken CXCR4, but has low homology to other CXCR family members.

Target

CXCR4 (Ser-324/Ser-325)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, ICC, IHC

Field of Research

Cell Signaling, Immunology, Oncoproteins

Format

Antigen Affinity Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylated

Molecular Weight

35,42,50,100

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Affinity purified rabbit serum

IHC Dilution

1:100

WB Dilution

1:1000

Specificity

The antibody detects bands at 35, 42, 50, and 100 kDa* corresponding to various post-translationally modified forms of CXCR4 on SDS-PAGE immunoblots of human Jurkat and PC3 cells stimulated with calyculin A. These bands co-migrate with similar bands detected with anti-CXCR4 (a.a. 322-329) and anti-CXCR4 (a.a. 328-338) antibodies.