Anti-CRMP2 (C-terminal region) Antibody

Our CRMP2 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat CRMP2 (C-terminal region) and is antigen affinity purified. It is great for use in ELISA, WB, ICC, IP.

Product Specifications

Background

CRMP2 (CRMP-62, TOAD-64, DRP-2) is a microtubule associated protein involved in neuron development and axon pathfinding. CRMP2 binds to tubulin heterodimers and promotes microtubule assembly. The overexpression of CRMP2 facilitates the rate of axonal growth, whereas the mutated form that lacks activity toward the microtubule assembly inhibits axonal growth in a dominant negative manner. Phosphorylation of CRMP2 regulates its activity and this type of regulation has been implicated in axon growth cone collapse induced by several repulsive cues. Cdk5 and GSK3 phosphorylation occurs downstream of the repulsive cue, Sema-3A. Several residues in CRMP2 are phosphorylated by GSK3 (Ser-518, Thr-514, and Thr-509), and a priming site (Ser-522) . These sites are conserved in human CRMP1 and CRMP4, but not in CRMP3 or CRMP5. The priming site is also phosphorylated by Cdk5. In contrast, ROCK phosphorylates Thr-555 leading to LPA, MAG, or Ephrin-A5 mediated growth cone collapse. Thus, CRMP2 phosphorylation status may be a critical element of pathways that control axon pathfinding.

Short Description

Synonyms

DRP-2, Toad-64, CRMP-62

Gene Name

DPYSL2

UniProt

Q16555

Reactivity

Human, Mouse, and Rat

Immunogen

CRMP2 synthetic peptide (coupled to carrier protein) corresponding to amino acids in the C-terminal region of human CRMP2. This sequence is conserved in rat and mouse CRMP2, and has high homology to the conserved site in CRMP1. It has 50% homology to CRMP3 and CRMP5.

Target

CRMP2 (C-terminal region)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, WB, ICC, IP

Field of Research

Neurodegenerative Disease, Alzheimer's Disease, Neurogenesis and Development

Format

Antigen Affinity Purified

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Molecular Weight

Precautions

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Shipping Conditions

Blue Ice

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Notes

Affinity purified rabbit serum

WB Dilution

1:1000

IP Dilution

1:100

Specificity

The antibody detects a 70 kDa* protein corresponding to the molecular mass of CRMP2 on SDS-PAGE immunoblots of human SKN-SH cells, mouse brain, and rat PC12 cells. This band on western blot can be specifically blocked using CRMP2 (C-terminal region) peptide (CX2165) .