Anti-CrkL (Tyr-207), Phosphospecific Antibody

The Crk family of adaptor proteins (Crk I, Crk II and CrkL) are Src Homology 2 (SH2) and Src Homology 3 (SH3) domain-containing proteins that form protein complexes important for transmiting signals downstream of tyrosine kinases. Both Crk II and CrkL are composed of a single SH2 domain, followed by two tandem SH3 domains. Crk II is also alternatively spliced to a minor product, Crk I, which is structurally and functionally more similar to the v-Crk oncogene. Both Crk II and CrkL are ubiquitously expressed and their SH domains are highly homologous, however both are required for mouse development and have distinct non-overlapping phenotypes in knockout mice. Phosphorylation may be important for regulating Crk activity. Crk II Tyr-221 (CrkL Tyr-207) phosphorylation is a negative regulatory site, while Crk Tyr-251 phosphorylation in the SH3 domain is a positive regulatory site. EGF stimulation induces phosphorylation of Tyr-251, which increases binding of Crk to the SH2 domain of Abl, and promotes transactivation of Abl.

Our CrkL (Tyr-207) phosphospecific primary antibody from PhosphoSolutions is rabbit polyclonal. It detects chicken, human, mouse, rat, and Xenopus CrkL (Tyr-207) and is antigen affinity purified. It is great for use in WB.

Phospho-Crk (Tyr-207) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-207 in human CrkL. This peptide sequence is well conserved in mouse and rat CrkL, and has 40% homology with the conserved site in Crk II (Tyr-221) . The site is not found in Crk I.

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

The antibody detects a 38 kDa* protein corresponding to the molecular mass of CrkL on SDS-PAGE immunoblots of human K562 cells stimulated with pervanadate. This reactivity is not observed after akaline phosphatase treatment.