Anti-Coronin-1B (C-terminus) Antibody

Coronins are highly-conserved F-actin binding proteins that play important roles in lamellipodial protrusion during various types of cell motility. In yeast, coronins regulate cytoskeletal changes through inhibition of Arp2/3 complex. Human coronins have been classified in three subgroups type I (coronin-1A, -1B, -1C), type II (coronin-2A, -2B), and type III (coronin-7) . These coronins have at least one large b-propeller region that mediates protein-protein interactions and type I and II coronins have coiled-coil regions involved in oligiomerization. Coronin-1B is ubiquitously expressed and localizes to the leading edge of cell protrusions in migrating fibroblasts. Both Coronin-1B and Coronin-1A interaction with Arp2/3 complex may be regulated by phosphorylation. PKC phosphorylates the N-terminus at Ser-2 , and this phosphorylation reduces interactions with Arp2/3 leading to diminshed cell motility.

Our Coronin-1B (C-terminus) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Coronin-1B (C-terminus) and is antigen affinity purified. It is great for use in WB, ICC.

Coronin-1B (C-terminus) synthetic peptide (coupled to carrier protein) corresponds to amino acids at the C-terminus of human coronin-1B. This sequence has high homology to the conserved site in rat and mouse coronin-1B, and less than 50% homology to similar regions in other coronins.

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

The antibody detects a 60 kDa* band corresponding to Coronin-1B on SDS-PAGE immunoblots of human A431 and HeLa, mouse brain, and rabbit spleen fibroblasts.